Table 1.
Predicted activation state of upstream transcriptional regulators in TCDD-treated AHR-positive differentiating ES cells.
| Differentiation day/ activation state | Regulator | z-Score | p-Value |
|---|---|---|---|
| Day 5 | |||
| Inhibited | APLNR | –2.1 | 5.05 × 10–7 |
| BMP2 | –2.4 | 1.57 × 10–6 | |
| BMP4 | –2.6 | 5.93 × 10–17 | |
| FGFR2 | –2.8 | 9.26 × 10–11 | |
| GLI2 | –2.4 | 3.31 × 10–7 | |
| Hedgehog | –2.7 | 1.42 × 10–19 | |
| MLL | –2.2 | 1.04 × 10–12 | |
| NFκB | –2.2 | 5.33 × 10–7 | |
| NKX2-5 | –1.1 | 1.37 × 10–8 | |
| SHH | –3.1 | 5.48 × 10–13 | |
| TGFB1 | –2.1 | 2.03 × 10–7 | |
| TNF | –2.8 | 1.75 × 10–7 | |
| WNT1 | –2.9 | 1.92 × 10–10 | |
| WNT3A | –2.9 | 7.34 × 10–11 | |
| Activated | GNL3 | 2.4 | 2.31 × 10–8 |
| POU5F1 | 2.3 | 1.85 × 10–19 | |
| RNF2 | 2.2 | 3.01 × 10–11 | |
| SOX2 | 2.3 | 1.78 × 10–20 | |
| Day 8 | |||
| Inhibited | APLNR | –2.4 | 1.86 × 10–9 |
| BMP4 | –3.3 | 2.81 × 10–23 | |
| BMPR1A | –2.7 | 2.87 × 10–9 | |
| CTNNB1 | –2.2 | 2.91 × 10–22 | |
| EPHB4 | –2.5 | 8.04 × 10–9 | |
| FGFR2 | –2.9 | 4.73 × 10–8 | |
| GLI2 | –2.4 | 7.45 × 10–12 | |
| MLL | –3.5 | 2.00 × 10–29 | |
| NKX2-5 | –0.5 | 2.98 × 10–8 | |
| STAT3 | –3.1 | 3.77 × 10–8 | |
| TGFB1 | –4.2 | 6.39 × 10–15 | |
| Tretinoin | –2.5 | 3.41 × 10–42 | |
| WNT11 | –2.2 | 2.71 × 10–8 | |
| Activated | GNL3 | 2.6 | 3.28 × 10–14 |
| PHC2 | 2.6 | 3.85 × 10–13 | |
| POU4F1 | 2.5 | 1.16 × 10–17 | |
| POU4F2 | 2.4 | 5.39 × 10–19 | |
| POU5F1 | 2.7 | 5.31 × 10–26 | |
| RNF2 | 3.1 | 1.27 × 10–16 | |
| SOX2 | 2.3 | 3.87 × 10–26 | |
| Day 11 | |||
| Inhibited | ARID4B | –2.1 | 8.03 × 10–7 |
| BMP2 | –2.5 | 3.10 × 10–15 | |
| BMP7 | –2.2 | 2.25 × 10–8 | |
| BMPR1A | –2.6 | 5.76 × 10–7 | |
| GLI3 | –2.4 | 1.48 × 10–11 | |
| HOXA9 | –2.2 | 9.81 × 10–10 | |
| MLL | –3.1 | 2.58 × 10–30 | |
| NKX2-5 | –0.7 | 5.94 × 10–8 | |
| SMO | –2.1 | 7.57 × 10–10 | |
| STAT3 | –2.6 | 4.71 × 10–7 | |
| TGFB1 | –3.4 | 1.48 × 10–10 | |
| Tretinoin | –2.8 | 1.63 × 10–36 | |
| Activated | KLF4 | 2.1 | 3.18 × 10–7 |
| NANOG | 2.1 | 2.68 × 10–18 | |
| OCT4 | 2.1 | 1.14 × 10–9 | |
| PHC2 | 2.4 | 8.70 × 10–11 | |
| POU4F2 | 2.2 | 7.99 × 10–15 | |
| POU5F1 | 2.5 | 1.19 × 10–25 | |
| RNF2 | 2.1 | 1.99 × 10–15 | |
| SOX2 | 2.3 | 1.07 × 10–22 | |
| Day 14 | |||
| Inhibited | ARID4A | –2.1 | 1.31 × 10–6 |
| ARID4B | –2.1 | 5.62 × 10–7 | |
| BMP2 | –2.5 | 4.31 × 10–10 | |
| EPHB4 | –2.1 | 6.34 × 10–11 | |
| GLI1 | –2.4 | 8.28 × 10–7 | |
| GSC | –2.1 | 2.56 × 10–6 | |
| HDAC | –2.2 | 3.26 × 10–10 | |
| HOXA9 | –2.2 | 1.61 × 10–8 | |
| miR-34a-5p | –2.2 | 2.72 × 10–8 | |
| MLL | –2.9 | 8.01 × 10–27 | |
| NKX2-5 | –0.7 | 2.98 × 10–8 | |
| SPRY1 | –2.2 | 3.88 × 10–7 | |
| STAT3 | –3.1 | 4.67 × 10–7 | |
| TGFB1 | –3.3 | 5.52 × 10–8 | |
| Tretinoin | –3.1 | 1.49 × 10–32 | |
| Activated | PHC2 | 2.4 | 5.07 × 10–11 |
| SOX2 | 2.2 | 4.68 × 10–22 |