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. 2013 Dec 5;8(12):e82580. doi: 10.1371/journal.pone.0082580

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© 2013 Schlegel et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A-F) Comparative analysis of PBMC stimulated with PMA/i for 20h or incubated in RPMI-1640 medium with the addition of Brefeldin A for 16 h in RA patients and healthy controls; A) Percentage of CD3^- non-T cells within IL-17⁺ lymphocytes (RA n = 20, healthy controls n = 20); B) Percentage of CD56⁺ NK cells within IL-17⁺CD3^- non-T cells; C) Percentage of IL-17⁺ cells within CD3^-CD56⁺ NK cells; D) Percentage of CD14⁺ monocytes in IL-17⁺CD3^- non-T cells within the lymphocyte gate and within the monocyte gate (FSC vs. SSC); E) Percentage of IL-17⁺ cells within CD3^-CD14⁺ monocytes within the monocyte gate; F) Percentage of CD11b⁺ cells within IL-17⁺CD3^- lymphocytes.

Data B)-F) PMBC of healthy controls (n = 8) and RA patients (n = 8).

*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant, column represents the mean and bars indicate 95% CI.