Table 1. Primers and probe sequences used for quantitative RT-PCR.
Gene | Accession number | Primer/probe | Position | Sequence |
---|---|---|---|---|
Uts2 | NM_019160 | Forward primer | 294 | GGCTCTCACTGGGCAAGATTC |
Reverse primer | 367 | GTTGCTTACGTTGTTTCCTGGTT | ||
Probe | 317 | ACACTGTACTGAGCCGTCTTTTGGCGA | ||
Gapdh | NM_017008 | Forward primer | 895 | CTACACTGAGGACCAGGTTGTC |
Reverse primer | 961 | CTGTGACTTCAACAGCA | ||
Probe | 919 | CATCAAAGGTGGAAGAATGG | ||
Actb | NM_031144 | Forward primer | 931 | GACAGGATGCAGAAGGAGATTACTG |
Reverse primer | 1031 | GAGCCACCAATCCACACAGA | ||
Probe | 969 | CACCATGAAGATCAAGATCATTGCTCCTCCT | ||
Uts2d | NM_198133 | Forward primer | 653 | ATGGAACTGAGAACTGCGTATC |
Reverse primer | 733 | AGACTTCAGGCGAGCATACA | ||
Uts2r | NM_020537 | Forward primer | 343 | GACTGGCACTTTGGAGATGT |
Reverse primer | 443 | CGTTCGCTGCTCATTATGGT |
Note: geNormTM house-keeping gene sequences are not publicly available