. Author manuscript; available in PMC: 2014 Dec 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Sep 19;940:10.1016/j.jchromb.2013.09.016. doi: 10.1016/j.jchromb.2013.09.016

Table 1.

Gradient program for the separation of free amino acids in plasma.

Time (min)	Conc. of channel (%)				Oven Temp (°C)

	A	B	C	D
0.0	97.8	2.2	0.0	0.0	42
2.0 – 29.0	96.1	3.9	0.0	0.0
38.0 – 45.0	90.0	10.0	0.0	0.0	38 at 42.1 min
48.0	80.0	10.0	0.0	10.0
57.0	43.5	16.5	0.0	40.0
60.0	32.9	17.1	0.0	50.0
65.0 – 95.0	32.0	18.0	0.0	50.0
95.10 – 98.0	0.0	20.0	80.0	0.0	42 at 97.1 min
98.1 – 113.0	0.0	80.0	20	0.0