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Differential scanning calorimetry profile for a 0.3 mM N-NFU solution in phosphate buffer. Origin was used to fit the data to a 2-peak, non-2-state model (MN2State). Values for Tm, ΔHcal and ΔvH were obtained from the fit and are listed in Tables 1 and 2. The lower melting point was initialized at 79.4 °C, which was obtained from a VTCD analysis.
