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. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: Biochemistry. 2013 Jul 15;52(29):10.1021/bi400320s. doi: 10.1021/bi400320s

Figure 2.

Figure 2

VTCD analysis of 10 μM N-NFU in 40 mM phosphate 100 mM NaCl buffer. Data were fit to equation 1 to yield values of ΔHυ, Tmsec, and ΔCp of 69.56 kcal/mol, 79.44 °C and 2.02 kcal/mol.K, respectively. Ellipticity data was directly used without converting to molar ellipticity units because the van't Hoff enthalpies are independent of such a factor. Tmsec was used as an initial value for Tm in the DSC data fitting routine.