Figure 6.

¹H-¹⁵N HSQC spectra of (A) ¹⁵N-C-NFU, (B) [¹⁴N-N-NFU + ¹⁵N-C-NFU] and (C) ¹⁵N-NFU. The C-terminal domain undergoes structural change in the presence of the N-terminal domain, as reflected by the peaks circled in red in (B). Cross peaks are also observed in spectrum (B) from the non-enriched N-NFU domain as a result of natural abundance ¹⁵N and provide an effective comparison of spectra for full-length NFU (C) relative to the sum of the individual domains (B). The cross-peaks circled in green in spectrum (C) highlight the chemical shifts of the full-length protein that are unique from the individual domains and in some cases overlaps with the peaks from the structure stabilized C-terminal domain. Chemical shifts for new cross-peaks identified in (B) and (C) are listed in Tables S2 and S3, respectively.