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Thermal profile for N-NFU. Data (dots) were converted to ΔG values by use of equation (3), and the Gibbs-Helmholtz equation, along with ΔHυ, Tmsec, and ΔCp, was used to form a plot of ΔG versus temperature (line). As expected, N-NFU is most thermally stable near human body temperature while melting of the protein is a favorable process at temperatures exceeding 79.4 °C.
