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Gibbs free energy plot illustrates a mixture of N-NFU and C-NFU domains to be most thermally stable near physiological temperature. The solid line represents the calculated data for N-NFU, and the dashed line the calculated data for C-NFU. Squares show the experimental data for N-NFU, and triangles show the experimental data for C-NFU. The vertical dashed lines indicate the fitted Tm's of C-NFU (315.0 K) and N-NFU (350.3 K).
