. Author manuscript; available in PMC: 2014 Jul 23.

Published in final edited form as: Biochemistry. 2013 Jul 15;52(29):10.1021/bi400320s. doi: 10.1021/bi400320s

Table 2.

ΔH_cal and ΔH_v for N-terminal NFU, C-terminal NFU, a mixture of N- and C-terminal domains, and full-length NFU determined by DSC.

	[NaCl] (mM)	ΔH_{cal 1} (kcal mol^-1)	ΔH_v1 (kcal mol^-1)	ΔH_{cal 2} (kcal mol^-1)	ΔH_v2 (kcal mol^-1)	ΔH_{cal 3} (kcal mol^-1)	ΔH_v3 (kcal mol^-1)	ΔH_{cal 4} (kcal mol^-1)	ΔH_v4 (kcal mol^-1)
N-NFU	100	134.6 ± 9.9	141.4 ± 4.2	93.2 ± 9.8	79.2 ± 1.6	-	-	-	-
N-NFU	0	59.1 ± 3.6	52.8 ± 1.4	66.2 ± 3.4	70.1 ± 4.1	-	-	-	-
C-NFU	100	-	-	-	-	-	-	-	-
C-NFU	0	-	-	-	-	-	-	-	-
N-NFU / C-NFU	100	42.0 ± 0.4	71.8 ± 5.8	40.9 ± 0.4	60.4 ± 5.3	68.2 ± 1.5	71.9 ± 4.9	289.4 ± 1.5	128.4 ± 38.4
N-NFU / C-NFU	0	28.3 ± 1.2	48.9 ± 2.9	38.2 ± 1.2	42.9 ± 3.4	28.2 ± 0.5	28.3 ± 2.6	194.4 ± 0.6	105.6 ± 2.5
NFU	100	94.0 ± 7.1	41.8 ± 8.2	20.1 ± 7.6	36.9 ± 3.3	125.7 ± 7.9	69.0 ± 45.1	37.9 ± 13.3	40.4 ± 2.7
NFU	0	29.6 ± 9.3	32.9 ± 11.6	57.3 ± 8.9	49.1 ± 29.9	28.3 ± 4.9	32.2 ± 15.4	102.8 ± 4.4	67.9 ± 1.9

By comparison with melting temperatures in Table 1, the enthalpic contribution from loss of secondary structure is listed in bold. In the cases of the N-NFU / C-NFU mixture and full-length NFU protein, two sets of enthalpies are observed due to the melting steps for the two independent domains.