. Author manuscript; available in PMC: 2014 Jul 23.

Published in final edited form as: Biochemistry. 2013 Jul 15;52(29):10.1021/bi400320s. doi: 10.1021/bi400320s

Table 3.

Circular dichroism analyses of N-terminal NFU, C-terminal NFU, a mixture of N- and C-terminal domains, and full-length NFU.

	N-NFU	C-NFU	N-NFU / C-NFU		NFU
	N-NFU	C-NFU	Experimental	Theoretical	NFU
α-helix	31%	30%	32%	30 %	32%
β-sheet	21%	16%	18%	18%	20%
Random Coil	48%	54%	50%	52%	48%

Data were obtained at 25 °C with a path length of 0.1 cm and fit with the K2D3 program,³² which yields a higher accuracy in β-strand prediction compared with its predecessor K2D.¹³ Theoretical values for the N-NFU/C-NFU mixture were calculated from the average values of the two isolated domains.