Table 4.
Melting temperatures of N-terminal NFU, C-terminal NFU, a mixture of N- and C- terminal domains, and full-length NFU determined by VTCD.
| [NaCl] (mM) | Tm1 (°C) | Tm2 (°C) | |
|---|---|---|---|
| N-NFU | 100.0 | 79.5 ± 0.4 | |
| 0.0 | 75.7 ± 0.1 | ||
| C-NFU | 100.0 | 59.4 ± 3.8 | |
| 0.0 | 60.7 ± 0.5 | ||
| N-NFU / C-NFU | 100.0 | 59.2 ± 0.5 | 78.6 ± 0.2 |
| 0.0 | 41.9 ± 1.5 | 77.2 ± 0.3 | |
| NFU | 100.0 | 67.8 ± 0.6 | 77.8 ± 0.9 |
| 0.0 | 50.2 ± 1.2 | 71.0 ± 0.4 |
The melting points were determined from molar ellipticity data fit with equations 1 or 2. As a result of the broad transitions observed for the C-NFU domain, Tm's were estimated according to a single transition and indicated with asterisk marks. In rows containing data collected on a mixture of N-NFU / C-NFU and the full-length NFU, the Tm1 and the Tm2 parameters refer to melting temperatures for the C-terminal and N-terminal domains, respectively.