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. 2013 Dec 6;8(12):e80128. doi: 10.1371/journal.pone.0080128

Figure 1. Env-SU induces the maturation of murine DC.

Figure 1

DC from wild-type C57Bl/6 mice were stimulated with LPS (2 µg/mL) or Env-SU (2 µg/mL). After 24 h of incubation, cells were analysed by cytofluorometry for MHC class II and CD11c surface markers expression. DC were harvested, washed in PBS with 3% FCS and 0.16% sodium azide and immunostained for MHCII (monoclonal IAb-PE antibody) and for CD11c (monoclonal CD11c-APC antibody) for 30 min at 4°C. The conjugated Abs used for staining were all obtained from BD Biosciences. A propidium iodide staining has been used to exclude dead cells. Double positive cells for CD11c and MHC-II (upper right quadrant) represents mature DC. Upper panel: Graphic distribution of cells according to immunostaining intensity for CD11c (abscissa) and MHCII (ordinate) without stimulation (left), after stimulation by LPS (center) or after stimulation by MSRV-Env (right). Lower panel: Corresponding percentage of cells in each quadrant, below each graphic. Results are representative for three experiments.