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. 2013 Dec 6;8(12):e80128. doi: 10.1371/journal.pone.0080128

Figure 2. Env-SU induced the production of pro-inflammatory cytokine in DC culture.

Figure 2

Murine Bone marrow DC were stimulated with LPS (2 µg/mL), PSB (100 µg/mL) and different amounts of Env-SU for 24 h. Culture supernatants were then analysed by ELISA for IL-6 (A), TNFα (B) and IL-12p70 (C) production. In (D), the specific inhibition of MSRV-Env stimulated DC by anti-Env neutralizing monoclonal antibody (GN-mAb_03) is shown, compared to an irrelevant anti-MSRV Gag (GN-mAb_12) or to diluents without antibody. IL-6 levels obtained in non-stimulated DC (medium) as negative control, or stimulated with LPS as positive control are also shown. IL-6 production was measured by ELISA in supernatants after 24 h of incubation (ELISA standard deviations <5%). These results are representative of three experiments realised under identical conditions.