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. 2013 Dec 6;8(12):e80128. doi: 10.1371/journal.pone.0080128

Figure 4. MSRV induced EAE like symptoms in C57Bl/6 mice.

Figure 4

Experiments were performed on 6–8 weeks-old C57BL/6 female mice. Mice were immunized subcutaneously with 200 µg of MOG 35–55 per animal emulsified in CFA containing 400 µg of Mycobacterium tuberculosis H37RA, or an emulsion containing 10 µg or 50 µg of Env-SU in IFA, or an emulsion containing 20 µg or 50 µg of full-length Env protein (in C), or MOG with IFA alone. Immediately thereafter and again 2 days later, the mice received an intraperitoneal injection of 200 ng of Bordetella pertussis toxin in PBS. Mice of IFA, CFA or Env groups were examined daily and clinical score were then monitored on the clinical scale of 0 to 6. Mean scores and standard deviation (A–B) or SEM (C) for each group of mice are presented. The values are representative for three experiments with Env-SU and more than three with full-length MSRV-Env (with protocol variants) that repeatedly showed similar induction of EAE.