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. Author manuscript; available in PMC: 2014 Jun 5.
Published in final edited form as: Nature. 2013 Nov 6;504(7478):163–167. doi: 10.1038/nature12652

Figure 2. EHMT1 is required for BAT-versus-muscle lineage specification.

Figure 2

a, Morphology of BAT from wild-type and Ehmt1 myf5 KO embryos at P1. Scale bar, 2.5 mm. b, H&E staining of wild-type and Ehmt1 myf5 KO BAT. Scale bar, 600 µm. Bottom: high magnification images. Scale bar, 30 µm. c, BAT weight from wild-type (n=14) and KO embryos (n=8). d, GO analyses of RNA-sequencing data. The Log2-fold changes in the expression of skeletal muscle (group M) and BAT (group B) genes are shown. e, Immunocytochemistry for MHC in C2C12 cells expressing indicated constructs under pro-myogenic culture conditions. Scale bar, 200 µm. f, Myogenin mRNA expression in e. n=3. g, ChIP assays using indicated antibodies. n=3. Error bars are s.e.m.; * P < 0.05, ** P <0.01, *** P <0.001.