Skip to main content
. Author manuscript; available in PMC: 2014 Jun 5.
Published in final edited form as: Nature. 2013 Nov 6;504(7478):163–167. doi: 10.1038/nature12652

Figure 3. EHMT1 controls BAT thermogenesis through stabilizing PRDM16 protein.

Figure 3

a, Cellular respiration in brown adipocytes expressing indicated constructs. n=6. b, BAT-selective gene expression in brown adipocytes expressing indicated constructs. n=3. c, Oil-Red-O staining of MEFs expressing indicated constructs under pro-adipogenic culture conditions. d, BAT-selective gene expression in c. n=3. e, Effects of EHMT1 mutants on PRDM16 transcriptional activites. n=3. f, PRDM16 protein levels in COS7 cells expressing indicated constructs. g, Regression analysis of the PRDM16 protein stability. n=3. h, Changes in rectal temperature during a cold challenge. n=4–5. Error bars are s.e.m.; * P < 0.05, ** P <0.01, *** P <0.001.