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The height of the color bars represents the fraction of each of the Ala codons in each dataset or sequence. The cDNAs used encoded the following AFPs; WF–hyp, LHS, SHS–skin, Atlantic snailfish AFP and cunner AFP. The number of non-AFP sequences used for each group is as follows: winter flounder, 70; longhorn sculpin, 10; cunner, 10 and four species in the snailfish family (Liparidae), 8. The accession numbers for these sequences are listed in Text S1.
