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Movement of the His181 side chain. (a) Cartoon diagram showing superposition of various subunits of the uracil complex. The uracil and active-site residues are shown as lines (gray, except for His181, which is colored by atom: C, magenta; N, blue; O, red). The region of the leucine-intercalation loop is colored red (labeled ‘Leu loop’). (b) Close-up view of His181 and uracil (both labeled) in the active site of various subunits.
