Table 4. Ultrastructural changes (Means ± S.D.) in Bt-SY63 and SY63 under ambient and elevated O3 (CCAP: the proportions of total chloroplast area in the entire cell area (%); PCAP: the proportion of total plastoglobule area in the entire cell area (%); SCAP: the proportion of total starch grain area in the entire cell area (%)).
|
CCAP (%)
|
PCAP (%)
|
SCAP (%)
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Variety | Treatment | 64-DAS | 85-DAS | 102-DAS | 64-DAS | 85-DAS | 102-DAS | 64-DAS | 85-DAS | 102-DAS |
| Bt-SY63 | A-O3 | 47.53±7.47 | 36.10±4.56 | None | 0.45±0.05 | 1.45±0.54b | 1.17±0.70 | 3.53±1.23 | 0.93±0.18 | 0.36±0.32 |
| F-O3 | 63.42±10.82 | 77.44±8.92 | None | 1.03±0.19 | 4.03±2.13a | none | 1.80±1.10 | 0.41±0.25 | none | |
| SY63 | A-O3 | 76.18±2.68 | 46.01±7.80 | 25.76±0.15 | 0.57±0.29 | 0.38±0.05b | 0.27±0.20 | 5.85±3.20 | 1.59±0.29 | 0.64±0.03 |
| F-O3 | 72.28±12.31 | 56.79±7.69 | 32.91±11.8 | 1.62±0.25 | 2.00±0.32ab | 1.02±0.55 | 2.32±1.60 | 1.09±0.06 | 1.09±0.13 | |
| Significance of two-way ANOVA | ||||||||||
| O3 | n.s. | ** | none | ** | * | none | * | ** | none | |
| Variety | * | n.s. | none | n.s. | * | none | n.s. | ** | none | |
| O3 × variety | n.s. | ** | none | n.s. | * | none | n.s. | * | none | |
A-O3, Ambient O3; E-O3, Elevated O3. Mean values and standard deviation of ten replicates are presented.
Two-way ANOVA: * p < 0.05, ** p < 0.01, n.s. = not significant.
None means an inability to determine the effect of the parameter owing to severely degenerated chloroplasts at this growth stage