Figure 6. Methylation profiles of WT and mutant ICRs from female and male embryonic germ cells.

Bisulfite sequencing of the CTCF site 2 and Sox-OctA and B region are shown for germ cells isolated from 14, 16, and 18 dpc ovaries and testes from heterozygous mutant embryos. WT and mutant alleles are denoted by white and grey backgrounds respectively. The parental origin of alleles is indicated by the symbol to the left of sequence graphical representations. Each block of bisulfite sequences was the result of 1–2 separate PCR reactions performed with DNA from 1×10³–1×10⁴ GFP-sorted germ cells obtained from 4–16 pooled gonads. DNAs from separate PCR reactions were cloned separately.