Table 1. Hair cell numbers do not return to normal levels following cessation of ethanol treatment.
O2 neuromast | Mi1 neuromast | |||
5 dpf | 10 dpf | 5 dpf | 10 dpf | |
Control | 13.1±2.3 | 12.4±0.9 | 12.8±1.8 | 11.1±0.92 |
1.00% EtOH | 10.2±2.0 | 9.4±1.6 | 10.6±2.0 | 9.1±1.0 |
1.50% EtOH | 8.9±2.1 | 8.3±1.0 | 9±2.3 | 8.2±1.1 |
Zebrafish were treated with ethanol from 2 days post-fertilization (dpf) to 5 dpf. Some animals were fixed and hair cell counts from the O2 and Mi1 neuromasts were obtained. Other embryos were raised in embryonic medium from 5 dpf to 10 dpf, fixed, and then hair cell counts were obtained. There were no significant differences in the number of hair cells within a specific condition between 5 dpf and 10 dpf larvae (p>0.1). Results are mean ± SD (n = 10-15 per condition).