Table 1. Primers used in real time analysis.
Gene | Name | Sequence (5’-3’) | Reference |
---|---|---|---|
CCR6 | CCR6-F | TGCAGAGGAAACAGTTAACAATTCA | [14] |
CCR6-R | CCAGTAAACCCAGGATACAGATGAC | ||
CCR7 | CCR7-F | TTCACTGATTACCCCACAGACAATA | [15] |
CCR7-R | AAGCAGATGAGGGAGTAAAAGGTG | ||
CCR9 | CCR9-F | TCAATCCCTTCCTGTATGTGTTTGT | [14] |
CCR9-R | GTCCGTGTCTGACATAACTGAGGAG | ||
CCR9B | CCR9B-F | AATATTTCCAACGTCTGAAACAGGA | [14] |
CCR9B-R | CTCACCCAGGACTTATCACACATTC | ||
CCR13 | CCR13-F | GTTCTGTACAACGTCTGGAAGGATT | [14] |
CCR13-R | ATGGCCAAAGGAAGTAGAAAGAAGA | ||
CXCR1 | CXCR1-F | CCTGATATCCAGAAGCTCTTTGTGT | [17] |
CXCR1-R | TTGCATCCAGCTCTATGATAATGAA | ||
CXCR4 | CXCR4-F | GTGCATGTGATCTACACCATC | [16] |
CXCR4-R | GAGCTGTGGCAAACACTATGT | ||
TLR-1 | TLR-1-F | CAGACGCCCTGTTGATGTTC | [ [56]]* |
TLR-1-R | CCTTCACAAGTTCCACCACG | ||
TLR-2 | TLR-2-F | GATCCAGAGCAACACTCTCAACAT | Accession number CCK73195* |
TLR-2-R | CTCCAGACCATGAAGTTGACAAAC | ||
TLR-3 | TLR-3-F | AGCCCTTTGCTGCCTTACAGAG | [57] |
TLR-3-R | GTCTTCAGGTCATTTTTGGACACG | ||
TLR-5mem | TLR-5mem-F | TTGACTTATCTTCCAACGGATTCA | [ [58]]* |
TLR-5mem-R | CTTTGAAATTGCTGAAACCAAATG | ||
TLR-7 | TLR-7-F | TACAGCTTGGTAACATGACTCTCC | [59] |
TLR-7-R | CAACTCTCTGAGACTTGTCGGTAA | ||
TLR-8a2 | TLR-8a2-F | CATCTATGTTCTCATCCAGCAACC | [ [59]]* |
TLR-8a2-R | GGTCCCCCTAATAGACAACCTCTT | ||
TLR-9 | TLR-9-F | TCTTCATAGAGCTGAAGAGGCCTCA | [ [60]]* |
TLR-9-R | GTTCCCACTGAGGAGAAGTGTTTT | ||
TLR-22 | TLR-22-F | TGGACAATGACGCTCTTTTACC | [61] |
TLR-22-R | GAGCTGATGGTTGCAATGAGG | ||
Pax5 | Pax5-F | ACGGAGATCGGATGTTCCTCTG | [54] |
Pax5-R | GATGCCGCGCTGTAGTAGTAC | ||
Blimp1 | Blimp1-F | AGCTGTCCAACCTCAAGGTCC | [54] |
Blimp1-R | TTGCGGCACACCTGGGCATTC | ||
MS4-A | MS4-A-F | GACAACAAACTGAACAAATGTCTGG | This paper |
MS4-A-R | GTAAAAGATGATCCCAGCACTGTCT | ||
mIgM | mIgM-F | CCTTAACCAGCCGAAAGGG | [54] |
mIgM-R | CCAACGCCATACAGCAGAG | ||
EF-1α | EF-1α-F | GATCCAGAAGGAGGTCACCA | [14] |
EF-1α-R | TTACGTTCGACCTTCCATCC |
* indicates that although the sequence had been previously reported and fully characterized, new primers for real time PCR were designed in this study.