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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: Mol Biochem Parasitol. 2012 May 8;184(2):10.1016/j.molbiopara.2012.04.009. doi: 10.1016/j.molbiopara.2012.04.009

Fig. 4.

Fig. 4

Immunolocalization of TgDHOD in intracellular tachyzoites. A. Parasites expressing endogenous TgDHOD tagged with three copies of c-myc or parasites exogenously expressing TgDHOD tagged with one copy of c-myc under the control of the tubulin promoter were visualized by deconvolution immunofluorescence with antibodies against myc (green) and the mitochondrial marker ATPase (red); nuclei were stained with DAPI. B. Dual staining of DHOD and the apicoplast marker Atrx (red); nuclei were stained with DAPI. C. Immunoblots of tachyzoite extracts from wild-type RH, endogenously tagged TgDHOD, and exogenously expressed TgDHOD were probed with affinity purified rabbit antibodies raised against TgDHOD-ALQD (left panel), antibodies raised against the myc-tag (middle panel), or mouse antibodies raised against TgDHOD-VSSM (right panel). An unknown antigen recognized by the rabbit antibodies is marked with an asterisk.