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. 2013 Dec 6;8(12):e82032. doi: 10.1371/journal.pone.0082032

Figure 4. The positive contribution of AtMIN7 to Pph-induced PR-1 transcript accumulation is independent of SA-signaling.

Figure 4

A) Col-0 or atmin7 plants were sprayed with water, infiltrated with Pph, or sprayed with 300 µM SA. PR-1 transcript levels were measured by qRT-PCR at 12 and 24 hours after spray or infiltration. Samples were normalized with the value for Pph in Col-0 at 24 hai set to 1 and the averages and standard deviations of data from four independent biological replicates is shown. Paired two-tailed t-tests indicate that transcript levels induced by SA spray did not vary significantly in Col-0 versus atmin7 at 12 or 24 hai (P≥0.7). B) Plants were treated as in (A) and 48 hours after spray or infiltration total protein from treated leaves was subjected to anti-PR-1 immunoblotting. Quantified data were normalized with the amount of PR-1 protein induced by SA in Col-0 set to 1. Average and standard deviations from three independent biological replicates are shown below the blot. The cross-reacting band above PR-1 and ponceau staining of RuBisCo indicate equal loading of samples.