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. 2013 Dec 6;8(12):e82769. doi: 10.1371/journal.pone.0082769

Figure 1. Microsatellite instability (MSI) and ATM gene mutations at introns in human gastric cancer cell lines.

Figure 1

(A) BAT26 (blue) and BAT25 (green) showed stable pattern in SNU-5 (upper) and unstable pattern in SNU-1 (lower). (B) ATM gene PCR products inintervening sequence (IVS6) (orange), IVS10 (blue), IVS20 (green) showed normal length in SNU-5 (upper) and shortening in SNU-1 (lower). (C) RT-PCR of ATM exon 6, exon 10, and exon 20 in gastric cancer cell lines. SNU-1 and 638 showed 22-bp deletion at exon 6. (D) Direct sequencing of IVS 6. SNU-5 has normal sequence and SNU-1 has 22 deletion at underlined sequences. (E) Immunohistochemistry of ATM protein in gastric cancer cell-lines. Negative in SNU-1, equivocal in SNU-638, mild positive in SNU-16 and strong positive in SNU-5. (F) Schematic representation of a 22-bp deletion of exon 6 of ATM (882del22).