Table 3.
Plasmids Used
| Plasmid | Description | Details | Source |
|---|---|---|---|
| pRS.416 | CEN, URA3 | Laboratory plasmid | |
| pRS.426 | 2µ, URA3 | Laboratory plasmid | |
| pBW0622 | GST-PAN1 CC | pEGKT∷GST-PAN1, aa792–1252 | Laboratory plasmid |
| pBW0623 | DBD-PAN1792–948 | pGBKT7∷Gal4 DBD-PAN1, aa 792–948 | Laboratory plasmid |
| pBW0910 | END3 | pRS.416∷END3 | Laboratory plasmid |
| pBW0923 | GST | pEGKT∷GST (CEN, URA3) | Laboratory plasmid |
| pBW1505 | HIS6-SYP1 µHD | pET28 CEN/URA∷HIS6-SYP1 µHD | Laboratory plasmid |
| pBW1649 | PAN1687–1190-TAP | pEGKT∷PAN1-TAP, aa687–1190 | Laboratory plasmid |
| pBW1659 | pGST-PAN1 LR2 | pEGKT∷GST-PAN1, aa360–690 | Laboratory plasmid |
| pBW1673 | DBD-PAN1687–1190 | pGBKT7∷Gal4 DBD-PAN1, aa687–1190 | Laboratory plasmid |
| pBW1680 | AD-BBC1 | pACTII∷Gal4 AD-BBC1, aa1096–1157 | This Study |
| pBW1681 | AD-CNA1 | pACTII∷Gal4 AD-CNA1 aa428–493 | This Study |
| pBW1684 | AD-END3 | pACTII∷Gal4 AD-END3, aa176–349 | This Study |
| pBW1685 | AD-KEL2 | pACTII∷Gal4 AD-KEL2, aa605–748 | This Study |
| pBW1858 | DBD-PAN1687–846 | pGBKT7∷Gal4 DBD-PAN1, aa687–846 | This Study |
| pBW1891 | HIS6-END3176–349 | pET28 CEN/URA∷HIS6-END3, aa176–349 | This Study |
| pBW1892 | HIS6-END3176–303 | pET28 CEN/URA∷HIS6-END3 aa176–303 | This Study |
| pBW1893 | HIS6-END3228–349 | pET28 CEN/URA∷HIS6-END3 aa228–349 | This Study |
| pBW2032 | HIS6-HΦA | pET28 CEN/URA∷HIS6-END3 HΦA | This Study |
| pBW2034 | HIS6-HΦR | pET28 CEN/URA∷HIS6-END3 HΦR | This Study |
| pBW2036 | HIS6-Hζ | pET28 CEN/URA∷HIS6-END3 Hζ | This Study |
| pBW2038 | HIS6-FH | pET28 CEN/URA∷HIS6-END3 FH | This Study |
| pBW2039 | HΦA | pRS.416∷END3 HΦA | This Study |
| pBW2040 | HΦR | pRS.416∷END3 HΦR | This Study |
| pBW2041 | Hζ | pRS.416∷END3 Hζ | This Study |
| pBW2042 | FH | pRS.416∷END3 FH | This Study |
| pBW2044 | HIS6-END31–349 | pET28 CEN/URA∷HIS6-END3, aa1–349 | This Study |
| pBW2046 | HIS6end3-1 | pET28 CEN/URA∷HIS6-end3–1 | This Study |
| pBW2199 | HIS6-E3R1:HΦR | pET28 CEN/URA∷HIS6-END3 E3R1:HΦR | This Study |
| pBW2201 | HIS6-E3R2:HΦR | pET28 CEN/URA∷HIS6-END3 E3R2:HΦR | This Study |
| pBW2203 | HIS6-E3R1:Hζ | pET28 CEN/URA∷HIS6-END3 E3R2:Hζ | This Study |
| pBW2205 | HIS6-E3R2:Hζ | pET28 CEN/URA∷HIS6-END3 E3R2:Hζ | This Study |
| pBW2207 | Hζ426 | pRS.426∷END3 Hζ | This Study |
| pBW2208 | E3R1:HΦR | pRS.416∷END3 E3R1:HΦR | This Study |
| pBW2209 | E3R2:HΦR | pRS.416∷END3 E3R2:HΦR | This Study |
| pBW2210 | E3R1:Hζ | pRS.416∷END3 E3R1:Hζ | This Study |
| pBW2211 | E3R2:Hζ | pRS.416∷END3 E3R2:Hζ | This Study |
| pBW2212 | HIS6-END3304–349 | pET28 CEN/URA∷HIS6-END3, aa304–349 | This Study |
| pBW2270 | HΦR.426 | pRS.426∷END3 HΦR | This Study |
| pBW2271 | E3R1:HΦR.426 | pRS.426∷END3 E3R1:HΦR | This Study |
| pBW2272 | E3R2:HΦR.426 | pRS.426∷END3 E3R2:HΦR | This Study |
| pBW2273 | E3R1:Hζ426 | pRS.426∷END3 E3R1:Hζ | This Study |
| pBW2274 | E3R2:Hζ426 | pRS.426∷END3 E3R2:Hζ | This Study |