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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Sep 21;1838(1):10.1016/j.bbamem.2013.09.011. doi: 10.1016/j.bbamem.2013.09.011

Figure 3.

Figure 3

The absorbance spectra of R. capsulatus superassembly during solubilization and purification in the best hydrophobic variant of the ganglio-TPAs (TPA-8), the lead ganglio-TPA (TPA-2), or two conventional detergents (DDM and LDAO) serving as positive or negative controls. (a) Resuspended membrane debris pelleted during ultracentrifugation, (b) solubilized supernatant from same procedure, and (c) IMAC-purified protein. Each detergent was used at 1.0 wt % for solubilization and at 1xCMC during IMAC steps. Data was collected on diluted samples (OD < 1.5), and spectra at the concentrations shown here were calculated using the dilution factor (normally 1:5 or 1:10).