Table 1.
Molecular weights (MW), critical micelle concentration (CMC), micellar hydrodynamic radii (Rh; mean ± SD, n = 3) and protein solubilization yields (SYs) for TPA-2, TPA-2 analogues and DDM.
| Detergent | MWa | CMC (mM) | CMC (wt %) | Rh (nm)b | SY (%)c |
|---|---|---|---|---|---|
| TPA-2 | 659.8 | ~3.6 | ~0.24 | 2.0 ± 0.06d | ~50 |
| TPA-6 | 673.8 | ~2.4 | ~0.16 | 2.3 ± 0.01d | ~70 |
| TPA-7 | 701.8 | ~1.3 | ~0.091 | 3.2 ± 0.06 | ~70 |
| TPA-8 | 715.9 | ~0.42 | ~0.030 | 4.1 ± 0.05 | ~80 |
| TPA-9 | 735.9 | ~0.14 | ~0.010 | 3.0 ± 0.01 | ~10 |
| TPA-10 | 687.8 | ~1.2 | ~0.083 | 2.7 ± 0.09 | ~50 |
| TPA-11 | 715.9 | ~0.56 | ~0.040 | 4.7 ± 0.05e | ~40 |
| DDM | 510.1 | ~0.17 | ~0.0087 | 3.5 ± 0.04 | ~70 |
a
Molecular weights
b
Hydrodynamic radius of micelles as determined at 0.5 wt % .
c
Solubilization yield of LHI-RC complex from the membrane.
d
TPA-2 and TPA-6 were used at 1.0 wt % to obtain strong signals.
e
Two forms of aggregates were observed with hydrodynamic radii of ~4.5 nm and ~270 nm (see Fig. 2)