Table 3.

Ethanol metabolism to acetaldehyde in the cytosolic fraction of uterine horn tissue from rats receiving an alcohol containing liquid diet.

Experimentala	Acetaldehyde (nmol/mg protein)
	Control	EtOH-treated
Ethanol only	1.39 ± 0.03	1.78 ± 0.05
Ethanol + NAD	1.67 ± 0.04b	0.43 ± 0.03g
Ethanol + NAD + HX	1.76 ± 0.13c,e	0.32 ± 0.01g,h
Ethanol + NAD + HX + allopurinol	0.13 ± 0.01c,d,f	0.27 ± 0.06g,i,j

^aIncubation mixtures containing cytosolic fraction (1.4–1.6 mg protein/mL), 0.14 M ethanol, and, when indicated, 0.25 mM hypoxanthine (HX) and 0.3 mM NAD⁺ in STKM buffer were conducted for 1 h at 37°C. Acetaldehyde was measured in the head space of each sample after adding 1 mL NaCl saturated solution. See Section 2 for details. Each result is the mean of three separate lots of pooled uterine tissue samples.

^b P < 0.01 when compared to “Ethanol only” (Control).

^c P < 0.001 when compared to “Ethanol only” (Control).

^d P < 0.001 when compared to “Ethanol + NAD + HX” (Control).

^e P > 0.05 when compared to “Ethanol + NAD” (Control).

^f P < 0.001 when compared to “Ethanol + NAD” (Control).

^g P < 0.001 when compared to “Ethanol only” (EtOH-treated).

^h P < 0.05 when compared to “Ethanol + NAD” (EtOH-treated).

ⁱ P > 0.05 when compared to “Ethanol + NAD + HX” (EtOH-treated).

^j P < 0.01 when compared to “Ethanol + NAD” (EtOH-treated).