Table 6.

Glutathione S-transferase activity (GST), glutathione reductase activity (GRed), and glutathione peroxidase activity (GPx) in uterine horn and liver tissue from rats receiving an ethanol containing liquid diet.

Group*	GST nmol conjugated GSH/min/mg protein	GRed nmol oxidized NADPH/min/mg protein	GPx nmol oxidized NADPH/min/mg protein
Uterine horn
Control	19 ± 1	107 ± 10	201 ± 2
EtOH	31 ± 1a	106 ± 5b	177 ± 2a
Liver
Control	148 ± 6	143 ± 6	677 ± 32
EtOH	152 ± 1b	223 ± 5a	664 ± 26b

*Glutathione S-transferase activity (GST) in uterine horn tissue and liver cytosol (3.6–3.9 mg protein/ml for uterine horn and 18.5–19.8 mg protein/mL for liver) was assayed as the thioether formation with CDNB and reading the absorbance at 412 nm. Glutathione reductase activity (GRed) in uterine horn tissue and liver cytosol (3.6–4.1 mg protein/mL for uterine horn and 18.2–19.5 mg protein/mL for liver) was determined by measuring the disappearance of NADPH at 340 nm. Glutathione peroxidase activity (GPx) in uterine horn tissue and liver cytosol (1.4–1.9 mg protein/mL for uterine horn and 8.9–11.2 mg protein/mL for liver) was analyzed in a reaction initiated by H₂O₂ and following the absorbance change at 340 nm. Each value is the mean ± S.D. from four separate samples (five animals each). See Section 2 for details.

^a P < 0.01 when compared to control.

^b P > 0.05 when compared to control.