Figure 4.

Luxol fast blue staining and MBP immunostaining for spinal cord and cerebral cortex. ((a)–(h)) C16 treatment prevented demyelination in spinal cord and cerebral cortex both at weeks 2 and 8 after immunization; Luxol fast blue staining; traverse section through the lumbar spinal cord; bar = 100 μm. At week 2 after immunization, normal rats (a), vehicle control rats (b) (inserted image depicting the inflammatory cells infiltrated demyelination area in anterior funiculus), 2 mg/per day C16 treated EAE rats (c) and C16 late treated EAE rats (d). At week 8 after immunization, vehicle control rats (e), 0.5 mg (f) and 2 mg (g)/day C16 treated EAE rats, and C16 late treated EAE rats (h). ((i)–(p)) C16 treatment prevented demyelination in spinal cord and cerebral cortex both at weeks 2 and 8 after immunization, MBP immunostaining, counterstained with hematoxylin ((i), (k), (m), and (o) coronal sections of motor cortex; (j), (l), (n), and (p) traverse sections through the lumbar spinal cord). At week 2 after immunization, normal rats ((i), (j)), vehicle control rats ((k), (l)), 2 mg/per day C16 treated EAE rats ((m), (n)) and C16 late treated EAE rats ((o), (p)).