Figure 3.

Effects of solvent fractions from Prunella vulgaris var. lilacina on LPS-induced pro-inflammatory mRNA expression and protein levels in RAW 264.7 cells. Cells were treated with fractions (50 μg/mL) and stimulated with LPS (1 μg/mL) for 12 h. (A,B) After incubation, cells were harvested for real-time RT-PCR to determine mRNA expression for (A) iNOS and (B) COX-2. (C) After incubation, cell lysates were used to determine iNOS and COX-2 protein levels via Western blot. Values show the means and standard deviations of three different experiments performed in triplicate. *p < 0.05 significantly different from the LPS-treated PBS group.