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. 2013 Nov 6;14(11):21965–21982. doi: 10.3390/ijms141121965

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(a) Two-dimensional schematic for a single channel used in this study consisting of a channel width (w) of 300 μm and length (l) of 40 mm. The height (not shown) is 130 μm. The two circular sections at the end of the channel mark the inlet and outlet; (b) A photograph of the microfluidic device in a polycarbonate holder. The holder positions the device with the glass side up for inspection by the confocal system and provides space on the bottom (PDMS) side for liquid delivery tubing access to the inlet and outlet holes. The w = 2 mm channels are connected to liquid delivery tubing and filled with a blue dye for visualization.