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. 2013 Nov 6;14(11):21983–21996. doi: 10.3390/ijms141121983

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Stomatal apertures (the ratio of width to length) of gene-silenced plants in response to ABA and H₂O₂. Leaves of control, individual gene-silenced (SpMPK1, SpMPK2, or SpMPK3) and combined gene-silenced (SpMPK1/SpMPK2 or SpMPK1/SpMPK2/SpMPK3) plants were incubated in stomatal opening solution for 2 h. Some of them were transferred to solutions containing 50 μM ABA or 50 mM H₂O₂ for 2 h and the others remained in the opening solution for control (CK). Stomata on the adaxial surface were observed by light microscopy. For each type of gene-silenced plants, at least 20 stomata from each sample of ten genes-silenced plants were measured. The data represent means ± SD. Asterisks indicate that the mean value is signficantly different from the control at p < 0.0001.