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. 2013 Nov 6;14(11):21983–21996. doi: 10.3390/ijms141121983

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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H₂O₂ production and CATs transcription levels of gene-silenced plants. (A) Detection of ABA-induced H₂O₂ production by DAB staining. Leaves of control, individual gene-silenced (SpMPK1, SpMPK2, or SpMPK3) and combined gene-silenced (SpMPK1/SpMPK2 or SpMPK1/SpMPK2/SpMPK3) plants were treated with 100 μM ABA for 2 h and transferred to 100 mg·mL⁻¹ DAB solution for 8 h. The presence of H₂O₂ in the leaves was visualized as a dark brown color; (B) The amount of H₂O₂ in leaves of gene-silenced plants. The data represent means ± SD of two leaves from each of six gene-silenced plants, respectively. Asterisk, p < 0.05; double asterisks, p < 0.01; (C) The transcription levels of H₂O₂ scavenging genes (CAT1 and CAT2) in VIGS plants treated with 100 μM ABA. The experiments are representative of 10 gene-silenced plants, respectively.