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. 2013 Nov 7;14(11):22102–22116. doi: 10.3390/ijms141122102

Figure 1.

Figure 1

SEPT12 interacts with α-tubulin and β-tubulin in male germ cell line. (A) Co-IP of FLAG-SEPT12 and α-tubulin. Lysates from transfected NTERA-2 d.D1 cells were immunoprecipitated (IP) with anti-FLAG antibody (left panel, lane3), anti-α-tubulin (right panel, lane 3), or a nonspecific control IgG (left and right panel, lane 2), followed by immuno-blotting (IB) with anti-α-tubulin or anti-FLAG antibody. Input protein (5%) was used as control of immuno-blotting (IB) in the transfected cell lysates; (B) Co-IP of FLAG-SEPT12 and β-tubulin. The data presents as (A).