Figure 3.

SEPT12 is co-localized with α- and β-tubulin at different steps of spermiogenesis in the SEPTIN12-transgenic mice. Immuno-fluorescence detection of SEPT12 and α-tubulin (A) or β-tubulin (B). Anti-EGFP antibody (A and B; Green), anti-α-tubulin (A, Red), anti-β-tubulin (B, Red) and DAPI (A and B; Blue). (a) early elongation stage; (b) elongating; (c) mature sperm. From left to right: DAPI (Blue), tubulin (Red), SEPT12 (Green) and merge of pictures. Enlarge figures are shown in right lower corner of (c). Scale bar : 5 μm.