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The effects of low-intensity pulsed ultrasound (LIPUS) on cell growth and Alkaline Phosphatase (ALP) activity in preosteoblast MC3T3-E1 cells. Cells were exposed to LIPUS at 30 mW/cm2 for 20 min followed by 0, 6, 24, and 48 h culture at 37 °C. The cell number (A) and ALP activity (B) were measured. Mock-treated cells served as a control. Data indicate the means ± SD for four different experiments.
