Table 1.
Pepper expressed sequence tags (ESTs) showing morphological change in VIGSed N. benthamiana plants.
| EST ID | BLASTX * | VIGSed phenotypes |
|---|---|---|
| Catalytic activity/ligase activity | ||
| KS01017F01 | Q8RU74_LYCES Dehydroquinate synthase (EC 4.6.1.3) | Stunted |
| KS26035G04 | Q5Z8K3_ORYSA Putative ZEITLUPE | Stunted |
|
| ||
| Catalytic activity/oxidoreductase activity | ||
| KS01002H05 | Q6S5A3_TROMA Sterol delta-7 reductase | Dead |
| KS13009F01 | Q9SVA9_ARATH Cytochrome P450-like protein | Leaf necrotic spot |
|
| ||
| Catalytic activity/transferase activity | ||
| KS13055B07 | Q5DKV0_TOBAC Adenosine kinase isoform 1T | Wrinkled leaf |
|
| ||
| Catalytic activity/hydrolase activity | ||
| KS23052B07 | chitinase/lysozyme [Nicotiana tabacum] | Pale green |
| KS08010H01 | probable pectate lyase P56-like [S. lycopersicum] | Stunted & wrinkled leaf |
| KS01052C01 | Q8H272_LYCES Metacaspase 1 (Fragment) | Wrinkled leaf |
|
| ||
| Binding/ion binding | ||
| KS12078F02 | calmodulin [Capsicum annuum] | Stunted |
| KS01006G03 | probable calcium-binding protein CML30-like [S. lycopersicum] | Stunted |
|
| ||
| Binding/protein binding | ||
| KS01047E07 | O04133_SOYBN SRC2 | Stunted |
|
| ||
| Binding/nucleic acid binding | ||
| KS01060G11 | Q93XV7_9ROSI Hydroxypyruvate reductase (EC 1.1.1.29) | Small leaf size |
| KS12047A04 | Q9XI07_ARATH F8K7.13 protein (Putative transcriptional regulatory protein) | Weak dwarf |
|
| ||
| Binding/others | ||
| KS01044F10 | BIP5_TOBAC Luminal binding protein 5 precursor (BiP 5) | Stunted |
| KS01057F02 | mannose-binding lectin [C. annuum] | Stunted |
| KS14050G06 | Q41424_SOLTU Chlorophyll a/b binding protein | Pale green |
|
| ||
| Structural molecule activity | ||
| KS10105B11 | Q9SCM3_ARATH 40S ribosomal protein S2 homolog (At3g57490) | Stunted & curling leaf |
| KS08008G04 | 60S ribosomal protein L10 [S. melongena] | Stunted & curling leaf |
|
| ||
| Transporter activity | ||
| KS24014C12 | Q93Y42_ARATH Coatomer delta subunit (Delta-coat protein) | Dead |
| KS01047B03 | Q9XF20_CAPCH Acyl carrier protein | Stunted |
| KS08017G06 | O80774_ARATH Hypothetical protein At2g34250 | Stunted & curling leaf |
|
| ||
| Transcription regulator activity | ||
| KS01043D02 | Q9XEE6_ARATH Hypothetical Cys-3-His zinc finger | Stunted |
| KS09041E04 | Q710C3_SPIOL Sigma factor (Fragment) | Pale green |
| KS09088A04 | Q7Y039_LYCES MADS-box protein 5 | Abnormal leaf |
| KS12065G09 | Q8RXK4_ARATH Hypothetical protein At4g38900 | Stunted |
|
| ||
| Unknown | ||
| KS20002B10 | later embryo abundant protein [Prunus dulcis] | Stunted |
| KS01047F02 | Q9C9T7_ARATH Hypothetical protein F25P22.17 | Stunted |
| KS01056A09 | No_hits | Complete dead |
| KS20014C01 | No_hits | Leaf local dead |
| KS07002E11 | AAD25142.1 (Hypothetical protein At3g24506) | Pale green |
| KS07012E06 | Q9FJZ7_ARATH Arabidopsis thaliana genomic DNA, chromosome 5, | Chlorosis |
| KS19065G03 | Q9C9V9_ARATH Putative golgi transport complex protein; 67058-70172 | Stunted & curling leaf |
*
Blast hit at http://genepool.kribb.re.kr/new/index.php-Solanaceae Gene Indices (six species) [19].