Figure 2.
Decreased lifespan in pdr-1 KO worms is exacerbated by Mn exposure. Two thousand five hundred L1 worms were treated with MnCl2 for 30 min, washed and then plated onto NGM plates spread with OP50 bacteria. Twenty worms were plated in triplicates per group. Worms were assessed for survival each day, and transferred to fresh plates every other day, until all worms had died. Wildtype worms: N2 strain; pdr-1 KO: pdr-1 (gk448) deletion. Compared to untreated WT animals, the other groups show a statistically significant leftward shift in lifespan (logrank test, p < 0.001), with one-way ANOVA analysis on median survival finding a significantly decreased lifespan in treated WT worms (p < 0.001) that is enhanced in the pdr-1 KO animals (p < 0.001).