Table 1.
Summary of Gene Set Enrichment Analysis (GSEA) analysis based on the Reactome database (http://www.reactome.org/ (accessed on 13 Novembre 2013)). Set size refers to the dimension of the pathway, and NTK (Normalized T-test of the kth gene set) is the observed value of the statistic as defined in the Graphite web tool [20]. Negative NTK values indicate pathways activated in veins, while positive values indicate pathways activated in arteries. It is worth noting that GSEA is known to have low statistical power; the suggested Q-value cut-off for identification of significant pathways is 0.25.
| Pathway | Set size | NTk | Q-Value |
|---|---|---|---|
| Complement cascade | 18 | −5.29 | 0 |
| Arachidonic acid metabolism | 11 | −3.09 | 0.044912281 |
| Glycosaminoglycan metabolism | 54 | −3.09 | 0.044912281 |
| MPS I—Hurler syndrome | 54 | −3.09 | 0.044912281 |
| MPS II—Hunter syndrome | 54 | −3.09 | 0.044912281 |
| MPS IIIA—Sanfilippo syndrome A | 54 | −3.09 | 0.044912281 |
| MPS IIIB—Sanfilippo syndrome B | 54 | −3.09 | 0.044912281 |
| MPS IIIC—Sanfilippo syndrome C | 54 | −3.09 | 0.044912281 |
| MPS IIID—Sanfilippo syndrome D | 54 | −3.09 | 0.044912281 |
| MPS IV—Morquio syndrome A | 54 | −3.09 | 0.044912281 |
| MPS IV—Morquio syndrome B | 54 | −3.09 | 0.044912281 |
| Biological oxidations | 56 | −2.75 | 0.106666667 |
| Cell surface interactions at the vascular wall | 54 | −2.75 | 0.106666667 |
| Keratan sulfate/keratin metabolism | 20 | −2.46 | 0.205977011 |
| G α (12/13) signaling events | 35 | −2.37 | 0.24 |
| Antigen presentation: Folding, assembly and peptide loading of class I MHC | 11 | −2.33 | 0.250980392 |
| Golgi associated vesicle biogenesis | 29 | −2.29 | 0.247017544 |
| Glutathione conjugation | 10 | −2.26 | 0.249756098 |
| Phase II conjugation | 23 | −2.26 | 0.249756098 |
| EGFR interacts with phospholipase C-γ | 17 | 2.12 | 0.273710692 |
| Ca-dependent events | 14 | 2.14 | 0.262564103 |
| Calmodulin induced events | 14 | 2.14 | 0.262564103 |
| CaM pathway | 14 | 2.14 | 0.262564103 |
| Cell-extracellular matrix interactions | 15 | 2.2 | 0.254184397 |
| PLCG1 events in ERBB2 signaling | 18 | 2.23 | 0.252121212 |
| DARPP-32 events | 12 | 2.26 | 0.249756098 |
| DAG and IP3 signaling | 15 | 2.29 | 0.247017544 |
| PLC-γ1 signaling | 15 | 2.29 | 0.247017544 |
| Amyloids | 18 | 2.33 | 0.250980392 |
| Telomere Maintenance | 31 | 2.46 | 0.192688172 |
| RNA polymerase I promoter opening | 18 | 2.65 | 0.131282051 |
| Chromosome maintenance | 53 | 2.75 | 0.1024 |
| Meiotic synapsis | 24 | 2.88 | 0.077575758 |
| Deposition of new CENPA-containing nucleosomes at the centromere | 21 | 2.88 | 0.077575758 |
| Nucleosome assembly | 21 | 2.88 | 0.077575758 |
| Packaging of telomere ends | 12 | 3.09 | 0.044912281 |
| Striated muscle contraction | 21 | 4.76 | 0 |
| Smooth muscle contraction | 19 | 6.13 | 0 |
| Muscle contraction | 36 | 7.25 | 0 |