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Image preprocessing for analysis. (a) Maximum intensity projections of two-photon images of a radial glia cell expressing farnesylated EGFP in vivo collected at 0, 5, and 10 minutes. (b) Same images after Sobel edge detection and binarization. (c) Binarized images underwent dilation with a 6-pixel radius. Pixel dilation helps eliminate artifactual discontinuities in the images (arrowhead in insets) resulting from the binarization step. Scale bar = 10 μm.
