Figure 5.

Comparing the relative sensitivity of the two motility measurement methods using images of normal radial glia cells (N = 10) before and after injection of 100 μM MK-801 into the ventricle of the tadpole to reduce motility. ((a), (b)) Example of glial motility before (a) and after (b) drug treatment. (c) Motility index changes measured using Method 1 from [15]. (d) Results using the boxcar-based Method 2. Each individual cell is depicted with the same color in (c) and (d). Note that Method 2 gives more consistent results. All values are normalized to the mean predrug motility index to facilitate comparison. Scale bar = 10 μm in upper panel and 2 μm in lower panel of (a).