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Tyrosine-hydroxylase immunostaining demonstrates high (A,C) and low (B,D) neuronal densities in one quadrant of the substantia nigra and in the locus coeruleus while tryptophan-hydroxylase immunostaining demonstrates high (E) and low (F) neuronal densities in the dorsal raphe nucleus. The medial longitudinal fasciculus is present in the lower border of the photomicrographs. Scale bar = 125 μm.
