Figure 7.

Efficient deamination of the BDF2 site does not require ADAR2’s dsRBDs. A Sequencing traces for RT-PCR products of edited regions in BDF2 for two minute reactions containing 150 nM of either full length human ADAR2 (hADAR2) or the isolated catalytic domain (hADAR2-D); edited adenosines indicated by a star. B Plot of product vs time for reaction of either hADAR2 (squares, dashed line) or hADAR2-D (circles, solid line) with BDF2 RNA (n = 3). C % editing of BDF2 RNA at 10 minutes at different enzyme concentrations. Error represents standard error (n = 3).