Skip to main content
. Author manuscript; available in PMC: 2013 Dec 8.
Published in final edited form as: Cell Rep. 2013 Oct 24;5(3):10.1016/j.celrep.2013.09.029. doi: 10.1016/j.celrep.2013.09.029

Figure 6. Trimming does not affect efficiency and fidelity of endogenous target silencing.

Figure 6

(A) pre-miR-430Ago2 hairpin is able to repress endogenous target genes independently of trimming. Cumulative distributions of fold changes between wild-type embryos and uninjected or injected MZdicer embryos for 122 validated miR-430 targets are shown. The cumulative distribution of non-target genes in wild type / MZdicer is plotted as a reference (grey line). See also Table S1.

(B) Venn diagram showing the number of miR-430 targets that are repressed in wild-type embryos or in MZdicer embryos injected with miR-430 duplex, pre-miR-430Ago2 hairpin or pre-miR-430Ago2 hairpin 3×PS compared to uninjected MZdicer embryos.

(C) Trimming has a negligible effect on target selection. Genes that are expressed >5 rpkm and over-repressed >2-fold by pre-miR-430Ago2 hairpin or pre-miR-430Ago2 hairpin 3×PS compared to miR-430 duplex are shown in red.