Skip to main content
. Author manuscript; available in PMC: 2014 Sep 12.
Published in final edited form as: Cell. 2013 Aug 29;154(6):1380–1389. doi: 10.1016/j.cell.2013.08.021

Figure 1. Effect of guide sequence extension on Cas9 activity.

Figure 1

(A) Schematic showing Cas9 with matching or mismatching sgRNA sequences targeting the human EMX1 locus. (B) SURVEYOR assay gel showing comparable modification of target 1 by sgRNAs bearing 20- and 30-nt long guide sequences. (C) Northern blot showing that extended sgRNAs are largely reverted to 20-nt guide-length sgRNAs in HEK 293FT cells.