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. Author manuscript; available in PMC: 2014 Sep 12.
Published in final edited form as: Cell. 2013 Aug 29;154(6):1380–1389. doi: 10.1016/j.cell.2013.08.021

Figure 6. Cas9 double nicking mediates efficient indel formation in mouse embryos.

Figure 6

(A) Schematic illustrating Cas9 double nicking targeting at the mouse Mecp2 locus. Representative indels are shown for mouse blastocysts co-injected with Cas9n-encoding mRNA and in vitro transcribed sgRNA pairs. (B) Efficient blastocyst modification is achieved at multiple concentrations of sgRNAs and wildtype Cas9 or Cas9n (titrating from 3 ng/uL to 100 ng/uL).