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. 2013 Dec 9;3:3456. doi: 10.1038/srep03456

Figure 5. IL-17A-induced resistance to docetaxel is dependent of ERK1/2 activation.

Figure 5

(A and C) MCF7 and BT20 cells were stimulated as described in Figure 4. When indicated, the MEK inhibitor U0126 was added at 10 μM 24 h before docetaxel. The cytotoxicity was determined using the Cytotoxicity Detection Kit (Roche). For each cell line, data are representative of 3 independent experiments performed in duplicates (* P < 0.05; ** P < 0.01). (B and D) Western blot analysis of phospho (pThr202/pTyr204) ERK1/2 and total ERK1/2 in MCF7 and BT20 cell lines untreated (medium) or treated with 10 μM U0126 inhibitor alone (U0126), 10 ng/ml of recombinant IL-17A (IL-17A) or 10 ng/ml of recombinant IL-17A + 10 μM U0126 inhibitor (IL-17A + U0126) for 30 min and 3 h.